For research use only
| Cat No. | ABC-TC212S |
| Product Type | Mouse Brain Cancer Cell Lines |
| Cell Type | Microglial |
| Species | Mouse-C57BL/6 |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Brain |
| Product Code | BV-2, BV 2, BV2 |
BV-2 Microglial Cells, derived from C57/BL6 mice and immortalized with v-raf/v-myc, model primary microglia in neurodegenerative disease research.
The BV-2 cell line is an immortalized microglial cell line derived from C57BL/6 mice. The cells are immortalized by v-raf/v-myc carrying J2 retrovirus. These cells retain microglial morphological (including a small cell body with branched processes) and functional characteristics. In in vitro culture, BV-2 cells show semi-adherent pattern, some cells may appear loosely attached or slightly suspended. Like other microglia, BV-2 cells can be activated by oxidative stress or inflammatory factors to release pro-inflammatory cytokines. Such stimuli are used to model neuroinflammatory processes associated with neurodegenerative diseases like Alzheimer’s disease and Parkinson’s disease. BV-2 cells are not tumorigenic in vivo and are primarily used as a non-tumorigenic model of microglial function. Besides, these cells mimic primary microglia by expressing surface markers such as CD11b and producing inflammatory mediators like TNF-α, IL-6, and nitric oxide when activated. Our murine cell lines include microglial cells, derived from the central nervous system, with a focus on the microglial cells origin, microglial cells location, and microglial cells culture conditions for neuroinflammation studies.
| Product Code | BV-2, BV 2, BV2 |
| Species | Mouse-C57BL/6 |
| Cat.No | ABC-TC212S |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Microglial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Brain |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Mouse Brain Cancer Cell Lines |
| Key Features | -Backed by AcceGen advanced technology |
BV-2 cells are widely used to study neuroinflammation, neurotoxicity, and microglial polarization in diseases like multiple sclerosis, ALS, and brain tumors. They serve as a screening platform for anti-inflammatory drugs, nanoparticle toxicity, and neuroprotective agents. Their ability to model M1/M2 activation states makes them ideal for investigating immune responses in neurodegeneration. BV-2 cells are also employed in infectious disease research (e.g., Zika, HIV-associated CNS disorders) due to their susceptibility to viral infection and cytokine profiling utility.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
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BV-2 cells are a murine microglial cell line commonly used as a model for studying microglial function and neuroinflammation. They offer a consistent and reproducible system for investigating the immune responses of microglia in the central nervous system.
Medium: Use high-glucose DMEM supplemented with 10% fetal bovine serum (FBS), L-glutamine, and antibiotics (penicillin-streptomycin).
Incubation: Maintain cells at 37°C in a humidified atmosphere with 5% CO₂.
Medium Renewal: Change the medium every 2-3 days to ensure optimal nutrient availability and waste removal.
Lipopolysaccharide (LPS): Often used to activate microglial cells and induce an inflammatory response.
Interferon-gamma (IFN-γ): Enhances the activation and pro-inflammatory response of microglia.
Tumor Necrosis Factor-alpha (TNF-α): Used to study inflammatory signaling pathways.
Microglial cells are the resident immune cells of the central nervous system. They play key roles in immune surveillance, phagocytosis, and inflammatory response, making them essential for studying neuroinflammation and CNS-related diseases.
