Primary Cells

B6129SF2/J Mouse Embryonic Fibroblasts

  • For research use only

Cat No.

ABC-TC3041

Product Type

Mouse Primary Cells

Cell Type

Fibroblast

Species

B6129SF2/J Mouse

Growth Conditions

37 ℃, 5% CO2

Source Organ

Embryo

Disease

Normal

Storage

Liquid Nitrogen

Mouse Embryonic Fibroblasts are isolated from day 13 mouse embryos of pathogen-free laboratory mice.

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Description

B6129SF2/J Mouse Embryonic Fibroblasts (MEFs) are derived from mid-gestation embryos of B6129SF2/J mice. These cells are isolated from healthy, pathogen-free embryos. These fibroblasts display adherent growth and a flattened, spindle-like morphology, making them suitable for studies of B6129SF2/J embryo fibroblast expression. MEFs are widely used as feeder layers for stem cell cultures due to their secretion of supportive growth factors and extracellular matrix proteins. They play critical roles in studies of cellular senescence, reprogramming, and developmental biology. MEFs express mesenchymal markers such as vimentin and fibronectin and exhibit a finite lifespan in vitro. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.

Product Code

B6129SF2/J Mouse Embryonic Fibroblasts, B6129SF2J MEFs, Mouse embryonic fibroblasts B6129SF2/J

Species

B6129SF2/J Mouse

Cat.No

ABC-TC3041

Product Category

Primary Cells

Size/Quantity

1 vial

Cell Type

Fibroblast

Growth Mode

Adherent

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Embryo

Disease

Normal

Biosafety Level

1

Storage

Liquid Nitrogen

Product Type

Mouse Primary Cells

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Application

  • B6129SF2/J Mouse Embryonic Fibroblasts are a versatile tool for developmental studies and cellular reprogramming research. They are commonly used as a feeder layer for mouse or human pluripotent stem cells, and as a model for exploring senescence-associated signaling and the cellular responses to genotoxic stress. They exhibit stable mesenchymal marker expression and demonstrate contact inhibition during culture. MEFs are also widely used in CRISPR screening platforms, epigenetic modification studies, and oxidative stress assays.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

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High Viability
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Precision and Reliability
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