For research use only
| Cat No. | ABC-TC3041 |
| Product Type | Mouse Primary Cells |
| Cell Type | Fibroblast |
| Species | B6129SF2/J Mouse |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Embryo |
| Disease | Normal |
| Storage | Liquid Nitrogen |
Mouse Embryonic Fibroblasts are isolated from day 13 mouse embryos of pathogen-free laboratory mice.
B6129SF2/J Mouse Embryonic Fibroblasts (MEFs) are derived from mid-gestation embryos of B6129SF2/J mice. These cells are isolated from healthy, pathogen-free embryos. These fibroblasts display adherent growth and a flattened, spindle-like morphology, making them suitable for studies of B6129SF2/J embryo fibroblast expression. MEFs are widely used as feeder layers for stem cell cultures due to their secretion of supportive growth factors and extracellular matrix proteins. They play critical roles in studies of cellular senescence, reprogramming, and developmental biology. MEFs express mesenchymal markers such as vimentin and fibronectin and exhibit a finite lifespan in vitro. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.
| Product Code | B6129SF2/J Mouse Embryonic Fibroblasts, B6129SF2J MEFs, Mouse embryonic fibroblasts B6129SF2/J |
| Species | B6129SF2/J Mouse |
| Cat.No | ABC-TC3041 |
| Product Category | Primary Cells |
| Size/Quantity | 1 vial |
| Cell Type | Fibroblast |
| Growth Mode | Adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Embryo |
| Disease | Normal |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Mouse Primary Cells |
| Quality Control | All cells test negative for mycoplasma, bacteria, yeast, and fungi. |
B6129SF2/J Mouse Embryonic Fibroblasts are a versatile tool for developmental studies and cellular reprogramming research. They are commonly used as a feeder layer for mouse or human pluripotent stem cells, and as a model for exploring senescence-associated signaling and the cellular responses to genotoxic stress. They exhibit stable mesenchymal marker expression and demonstrate contact inhibition during culture. MEFs are also widely used in CRISPR screening platforms, epigenetic modification studies, and oxidative stress assays.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).