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Tumor Cell Lines

BT-549

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The BT-549 line was isolated in 1978 by W.G. Coutinho and E.Y. Lasfargues. Source tissue consisted of a papillary, invasive ductal tumor which had metastasized to 3 of 7 regional lymph nodes.
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Product Code

BT 549; BT.549; BT549

Species

Human

Cat.No

ABC-TC0087

Product Category Tumor Cell Lines
Size/Quantity

1 vial

Cell Type

Epithelial

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Breast

Disease

Ductal Cancer

Biosafety Level

1

Storage

Liquid Nitrogen

Product Type

Human Breast Cancer Cell Lines

Description

BT-549
BT-549 is a human breast carcinoma cell line originally isolated from the breast tissue of a 72-year-old white female patient with invasive ductal carcinoma accompanied by lymph node metastasis. BT-549 cells exhibit epithelial-like morphology and adherent growth characteristics. The cell population is polymorphic, containing epithelial-like components as well as multinucleated giant cells. Stemline karyotype analysis reveals that BT-549 cells are aneuploidy, with a modal chromosome number of 78 (ranging from 73 to 80), aincluding aberrations involving the X chromosomes.
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Citation

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Application

  • The BT-549 cell line has become a valuable asset in biological science, particularly in cancer research and 3D cell culture applications. Its significance lies in its relevance to ductal carcinoma, providiing insights into disease mechanisms and potential therapeutic targets. As a pivotal model in cancer research, BT-549 cells facilitate a deeper understanding of carcinoma development and the exploration of innovative treatment strategies. Moreover, their suitability for 3D cell culture makes them an ideal tool for mimicking the complex tumor microenvironment, enabling more physiologically relevant studies of drug responses and tumore progression.

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