C57BL/6 Mouse Lymphatic Fibroblasts

Description

C57BL/6 Mouse Lymphatic Fibroblasts are primary cells isolated from the perilymphatic connective tissue of pathogen-free laboratory mice, serving as a key model for studying C57BL/6 lymphatic fibroblast function and mouse lymphatic tissue dynamics. They primarily reside in the stromal compartments of lymph nodes, including the subcapsular sinus, medullary cords, and paracortical regions. Following primary culture, these cells are cryopreserved. The cells regulate lymph node structural integrity, immune cell trafficking, and antigen transport. Studies have highlighted their critical roles in secondary lymphedema, chronic lymph node inflammation, and tumor metastasis. Phenotypic validation confirms characteristic spindle-shaped morphology and expression of fibroblast-specific protein 1 (FSP1/S100A4). For optimal performance, repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.