C57BL/6 Mouse Mammary Epithelial Cells

Description

C57BL/6 Mouse Mammary Epithelial Cells are primary cells isolated from the mammary tissue of pathogen-free laboratory mice. They primarily reside in the terminal end buds (TEBs) of mammary ducts and the lobuloalveolar units during pregnancy/lactation. These cells serve as an important model for breast cell research. Following primary culture, these cells are cryopreserved. The cells are capable of self-renewal and maintain critical mammary gland functions including milk synthesis/secretion, ductal branching morphogenesis, and hormonal responsiveness to estrogen and progesterone. Phenotypic validation confirms expression of epithelial markers E-cadherin and ZO-1, demonstrating intact cell polarity and junctional complexes. For optimal performance, repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.