For research use only
| Cat No. | ABC-TC0389 |
| Product Type | Human Leukemia Cell Lines |
| Cell Type | Myeloblastic |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Peripheral Blood |
| Disease | Acute Promyelocytic Leukemia |
| Product Code | HL 60; HL.60 HL60; Human Leukemia-60 |
HL-60 cell line is a human promyelocytic leukemia model widely applied in hematopoiesis, differentiation, leukemia biology, and therapeutic drug testing.
HL-60 is a human promyeloblast cell line originally isolated from the peripheral blood of a 36-year-old Caucasian female diagnosed originally as acute promyelocytic leukemia, later reclassified as AML‑M2 (maturing AML). HL-60 exhibits lymphoblast-like morphology and grow in suspension. Karyotypically, HL-60 is pseudodiploid with modal number 43-46. While predominantly pseudodiploid, a small percentage of cells (around 6. 2%) exhibit a 2S component, adding to the complexity of their genomic makeup. This cell line expresses key myeloid differentiation markers such as CD33 and CD11b but lacks the PML-RARA fusion protein that is commonly present in classical APL cell. HL-60 is tumorigenic in SCID mice, where it can form granulocytic sarcomas following subcutaneous injection. HL‑60 retains responsiveness to differentiation agents—like DMSO, ATRA, vitamin D₃, PMA or GM‑CSF—facilitating granulocytic, monocytic/macrophage, or eosinophil lineage commitment.
| Product Code | HL 60; HL.60 HL60; Human Leukemia-60 |
| Species | Human |
| Cat.No | ABC-TC0389 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Myeloblastic |
| Growth Mode | Suspension |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Peripheral Blood |
| Disease | Acute Promyelocytic Leukemia |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Leukemia Cell Lines |
HL-60 cells are a widely used model in hematological and cancer research. They are instrumental in studying leukemic cell proliferation, differentiation, apoptosis, and the mechanisms of myeloid lineage commitment. Their responsiveness to various differentiation agents makes them an ideal platform for evaluating the efficacy of anticancer drugs and chemotherapy efficiency. Additionally, HL-60 cells are valuable for investigating phagocytosis, calcium signaling, and the production of extracelluler vesicles (EV). Furthermore, HL-60 cells serve as an essential model system for immunological research and vaccine development, serving as a versatile in vitro system for elucidating cellular and molecular pathways in myeloid biology.
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