For research use only
| Cat No. | ABC-TC0389 |
| Product Type | Human Leukemia Cell Lines |
| Cell Type | Myeloblastic |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Peripheral Blood |
| Disease | Acute Promyelocytic Leukemia |
| Product Code | HL 60; HL.60 HL60; Human Leukemia-60 |
HL-60 cell line is a human promyelocytic leukemia model widely applied in hematopoiesis, differentiation, leukemia biology, and therapeutic drug testing.
HL-60 cells are a human myeloid leukemia cell line originally established from the peripheral blood of a 36-year-old female patient diagnosed with acute promyelocytic leukemia and subsequently characterized as a myeloblastic leukemia model. The cells grow in suspension and have been extensively studied for their capacity to undergo myeloid differentiation in response to chemical and biological stimuli. Treatment with agents such as dimethyl sulfoxide (DMSO) or all-trans retinoic acid (ATRA) can induce differentiation toward a neutrophil-like phenotype, while other stimuli can promote monocytic differentiation. This differentiation capacity makes HL-60 cells a widely used experimental model for investigating myeloid cell biology, leukemia, differentiation, and cellular responses.
| Product Code | HL 60; HL.60 HL60; Human Leukemia-60 |
| Species | Human |
| Cat.No | ABC-TC0389 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Myeloblastic |
| Growth Mode | Suspension |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Peripheral Blood |
| Disease | Acute Promyelocytic Leukemia |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Leukemia Cell Lines |
HL-60 cells can be used for research on myeloid differentiation, leukemia biology, apoptosis, proliferation, and drug response. Their well-established differentiation response makes them particularly useful for generating neutrophil-like or monocyte-like cells for studies of innate immune functions, including phagocytosis, oxidative responses, and inflammatory signaling. HL-60 cells can also be used to evaluate the effects of anticancer compounds, differentiation-inducing agents, and other experimental treatments on leukemic cell survival and phenotype. Because differentiation protocols can produce cells with different phenotypic and functional characteristics depending on the inducing agent and culture conditions, differentiated HL-60 cells should be interpreted as experimental myeloid models rather than direct substitutes for primary mature granulocytes.
Research using HL-60 cells focuses on myeloid differentiation, acute myeloid leukemia, neutrophil biology, monocytic differentiation, apoptosis, inflammatory signaling, phagocytosis, oxidative burst, and therapeutic drug response. HL-60 cells are widely used to study the molecular mechanisms underlying leukemic cell proliferation and terminal differentiation, with DMSO and ATRA being commonly used approaches for generating neutrophil-like phenotypes. The model can also support investigations of innate immune responses and myeloid cell function, although the phenotype and functional properties of differentiated cells depend on the differentiation protocol used.
Cell Recovery(Thaw and recover the cryopreserved HL-60 cells under recommended suspension-culture conditions.) → Cell Culture(Maintain the cells in suspension and expand them to the required experimental density.) → Experimental Treatment(Expose HL-60 cells to differentiation agents, anticancer compounds, inflammatory stimuli, or other experimental conditions.) → Myeloid Differentiation(Induce neutrophil-like or monocytic differentiation using an appropriate differentiation protocol.) → Phenotypic Assessment(Evaluate morphological changes and lineage-associated cellular markers.) → Functional Analysis(Assess phagocytosis, oxidative responses, apoptosis, proliferation, or other myeloid-associated functions.) → Molecular Analysis(Analyze gene expression, protein expression, signaling pathways, and differentiation-associated molecular changes.) → Drug Response Analysis(Evaluate treatment-associated effects on HL-60 proliferation, survival, differentiation, or cellular function.)
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
