+1 862-686-2696
Featured Products
Primary Cells
Immortalized Cell Lines
Stem Cells
Tumor Cell Lines
Transfected Stable Cell Lines
Exosomes
MicroRNA Agomir/Antagomir
Cell Culture Media, kits & Reagents
Explore Products
Cell
Cell
MicroRNA
MicroRNA
Test and Analysis
Test and Analysis
Primary Cells

Human Keratinocytes, Adult

  • For research use only

Cat No.

ABC-TC3030
Product Type

Keratinocyte
Cell Type

Keratinocyte
Species

Human
Growth Conditions

37 ℃, 5% CO2
Source Organ

Skin
Disease

Normal
Storage

Liquid Nitrogen

Human Keratinocytes, Adult provide an ideal model for skin biology, wound healing, and drug testing, with no murine feeder layer, at early passage.

Product Image
  • Human Keratinocytes, Adult
Get a Quote
Home | Primary Cells | Human Primary Cells | Others | Human Keratinocytes, Adult

Description

Human Keratinocytes, Adult are isolated from healthy donor skin tissue (epidermis), specifically from the basal layer of the skin, maintaining characteristic cobblestone morphology and stratified growth potential in culture. These cells play a critical role in forming the epidermal barrier and protecting against mechanical, chemical, and microbial damage. These primary keratinocytes express standard keratinocyte markers (cytokeratin 5, 14 in basal layers; cytokeratin 1, 10 in differentiated layers) and demonstrate proper calcium-induced differentiation capacity. The cells exhibit physiological proliferation rates with balanced expression of epidermal growth factor receptors and cell cycle regulators (p63, Ki-67). They retain native barrier function properties and respond appropriately to wound healing stimuli (TGF-α, IL-1α). They are used in studies of skin disorders such as psoriasis, squamous cell carcinoma, and wound healing.

Species

Human
Cat.No

ABC-TC3030
Product Category

Primary Cells
Size/Quantity

1 vial
Cell Type

Keratinocyte
Growth Mode

Adherent
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Skin
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Keratinocyte
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Application

  • Human keratinocytes are widely used in scientific applications. In wound healing and some pathogenic conditions, growth factors and cytokines such as IL-1, TNF-α, TGF-α, TGF-β, and IFN-γ affect keratinocyte activation. Keratinocytes respond to signals from fibroblasts, such as TGF-β, by expressing K5 and K14, which leads to the restoration of their healthy basal phenotype. The differentiation of keratinocytes is mediated through the involvement of the mitogen-activated protein kinase (MAPK) pathway, which is influenced by factors such as epidermal growth factor (EGF), TNF, and calcium influx. Keratinocytes are also involved in the immune signaling pathway, as they express Toll-like receptors (TLRs) that respond to pathogen-associated molecular patterns (PAMPs). Toll-like receptors are expressed in human keratinocytes. TLR3, 4, 5 ligand stimulation can trigger an immune response in keratinocytes.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

  • Online Inquiry
  • Product Reviews

Inquiring Human Keratinocytes, Adult

We know how valuable your research is to you, but are you wondering what you can expect to pay for quick accurate results every time? Fill out a request in the form below and we’ll get back to you within 24 hours with a quote.
High Viability
To succeed in cell culture
Precision and Reliability
To support a consistent result
Customization Options
Tailed to your research

Tags

Let's Advance Life Science Research Together

Contact Us
AcceGen Scroll Top Button