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Immortalized Cell Lines

Immortalized Human Fibroblasts

  • For research use only

Cat No.

ABI-TC052D
Product Type

Immortalized Cell Line
Cell Type

Fibroblast
Species

Human
Growth Conditions

37 ℃, 5% CO2
Source Organ

Connective Tissue
Disease

Normal
Storage

Liquid Nitrogen

Immortalized human fibroblasts from foreskin show spindle shape, SV40-transformed, express fibronectin/vimentin,ideal for dermal research and cell-matrix.

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  • Immortalized Human Fibroblasts
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Home | Immortalized Cell Lines | Immortalized Human Fibroblasts

Description

Immortalized human fibroblast cell line is derived from normal human dermal fibroblasts and genetically modified to bypass replicative senescence through stable expression of SV40 large T antigen. The cells exhibit a spindle-shaped morphology typical of fibroblasts and grow as an adherent monolayer. They show expression of fibroblast markers such as vimentin, FSP1 (fibroblast-specific protein 1), and PDGFR-β. They retain key functional properties including ECM secretion, wound healing migration, and cytokine responsiveness. Immortalized human fibroblasts are commonly used in studies of fibrosis, tissue remodeling, aging, cancer-associated fibroblasts (CAFs), and inflammatory skin disorders. Their extended lifespan enables reproducible long-term experiments without the variability of primary fibroblasts.

Product Code

Immortalized Human Fibroblasts, Human Immortalized Fibroblasts, hFB, Immortalized Human Fibroblast Cells
Species

Human
Cat.No

ABI-TC052D
Product Category

Immortalized Cell Lines
Size/Quantity

1 vial
Cell Type

Fibroblast
Growth Mode

Adherent
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Connective Tissue
Disease

Normal
Storage

Liquid Nitrogen
Product Type

Immortalized Cell Line
Immortalization Method

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
Quality Control

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Application

  • Immortalized human fibroblasts are widely used in wound healing research due to their collagen secretion, which aids in elucidating repair mechanisms. They are essential in tissue engineering and regenerative medicine, supporting novel strategies for tissue repair. Additionally, these fibroblasts facilitate the growth and establishment of induced pluripotent stem (iPS) cells, advancing understanding of cell reprogramming and regenerative therapies.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Frequently Asked Questions

  • What is an immortalized cell line?

    An immortalized cell line is composed of cells that have been genetically or chemically modified to proliferate indefinitely in vitro. These cells bypass normal senescence while retaining many key phenotypic and functional characteristics relevant to research applications, making them suitable for long-term and reproducible research.

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High Viability
To succeed in cell culture
Precision and Reliability
To support a consistent result
Customization Options
Tailed to your research

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