Immortalized Human Fibroblasts

Description

The immortalized human fibroblast cell line is derived from neonatal foreskin of healthy Caucasian male donors. It shows typical spindle-shaped morphology and grows as an adherent monolayer under standard conditions (37°C, 5% CO₂) in fibroblast-specific culture medium usually supplemented with 10% FBS and essential growth factors. The line is immortalized by lentiviral SV40 Large T antigen transduction, which inactivates tumor suppressors p53 and Rb, confirmed by qPCR. It maintains near-diploid chromosomal stability, though minor alterations may occur. Fibroblast-specific markers such as vimentin and fibronectin are expressed, confirming lineage. These cells are non-tumorigenic and non-metastatic but require cautious handling due to SV40 immortalization. The cell line is tested free of HIV, HPV, EBV, mycoplasma, and fungi.

Application

• Immortalized Human Fibroblasts have broad applications in various fields of research. They are commonly used in the studies of wound healing, where their ability to secrete collagen proteins aids in understanding the underlying mechanisms. Additionally, these fibroblasts play a vital role in tissue engineering and regenerative medicine, contributing to the development of innovative approaches for repairing damaged tissues. Furthermore, they provide valuable support in the growth and establishment of induced pluripotent stem (iPS) cells, contributing to a deeper understanding of cell reprogramming and regenerative medicine.