The immortalized human fibroblast cell line is derived from neonatal foreskin of healthy Caucasian male donors. It shows typical spindle-shaped morphology and grows as an adherent monolayer under standard conditions (37°C, 5% CO₂) in fibroblast-specific culture medium usually supplemented with 10% FBS and essential growth factors. The line is immortalized by lentiviral SV40 Large T antigen transduction, which inactivates tumor suppressors p53 and Rb, confirmed by qPCR. It maintains near-diploid chromosomal stability, though minor alterations may occur. Fibroblast-specific markers such as vimentin and fibronectin are expressed, confirming lineage. These cells are non-tumorigenic and non-metastatic but require cautious handling due to SV40 immortalization. The cell line is tested free of HIV, HPV, EBV, mycoplasma, and fungi. 
Inquiring Immortalized Human Fibroblasts
We know how valuable your research is to you, but are you wondering what you can expect to pay for quick accurate results every time? Fill out a request in the form below and we’ll get back to you within 24 hours with a quote.
High Viability
To succeed in cell culture
Precision and Reliability
To support a consistent result
Customization Options
Tailed to your research
