Immortalized Cell Lines

Immortalized Human Skin Fibroblast – SV40

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Immortalised Human Skin Fibroblasts are Immortalised through infection with recombinant lentiviruses carrying SV40 Large T antigen
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Species

Human

Cat.No

ABI-TC301D

Quality Control

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Product Category Immortalized Cell Lines
Size/Quantity

1 vial

Cell Type

Fibroblast

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Skin

Disease

Normal

Biosafety Level

2

Storage

Liquid Nitrogen

Product Type

Immortalized Cell Line

Immortalization Method

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

Description

Immortalised Human Skin Fibroblasts are Immortalised through infection with recombinant lentiviruses carrying SV40 Large T antigen

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Citation

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Application

  • For research use only

Frequently Asked Questions

  • Is coating the flask with Poly-L-lysine essential, or can the cells still proliferate on an untreated flask?

    Coating with Poly-L-lysine (PLL) is mainly recommended to improve cell adhesion, particularly for cell lines with poor adhesion.

  • What is the origin of Immortalized Human Skin Fibroblasts, and how were they immortalized?

    ABI-TC301D cells are derived from dermal fibroblasts of the skin dermis and have been immortalized through SV40T transfection.

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