For research use only
| Cat No. | ABC-TC600S |
| Product Type | Human Lung Cancer Cell Lines |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Disease | Lung Cancer |
| Product Code | LCLC97TM1 |
LCLC-97TM1: Human large cell lung carcinoma cell line. Epithelial, adherent, integrates 1-2 copies of HPV16.
LCLC-97TM1 is a human non‑small‑cell lung cancer (NSCLC) cell line derived from a large cell lung carcinoma (LCLC) of a 44-year-old Caucasian male patient, originally xenografted into nude mice and subsequently cultured in vitro. Established from the first tumor passage, these adherent cells exhibit epithelial-like morphology with dense islet formation and indistinct borders. These cells contain large nuclei with prominent nucleoli and evenly dispersed chromatin, reflecting the undifferentiated phenotype. They do not attain full confluency in routine culture. LCLC-97TM1 displays a complex, abnormal karyotype indicative of high genomic instability typical of LCLC. They harbors a homozygous KRAS p.G12V activating mutation and heterozygous TP53 frameshift mutation (p.L35Cfs*9), hallmarks of NSCLC pathobiology. LCLC-97TM1 retains tumorigenicity in nude mice. This cell line serves as a relevant in vitro and in vivo model for investigating LCLC pathogenesis, tumor progression, and responses to therapeutic agents in non-small cell lung cancer (NSCLC).
| Product Code | LCLC97TM1 |
| Species | Human |
| Cat.No | ABC-TC600S |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Disease | Lung Cancer |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Lung Cancer Cell Lines |
LCLC‑97TM1 serves as a robust in vitro and in vivo model for studying large cell lung carcinoma (LCLC)—a subtype of NSCLC. It is particularly suitable for interrogating KRAS-driven oncogenic signaling, TP53-related dysregulation, genomic instability, and drug resistance mechanisms. The line supports xenograft models to evaluate candidate therapeutics and tumor response in immunodeficient mice.Its non-confluent, epithelioid growth and molecular profile make it suitable for high-content screening, KRAS-targeted agent testing, and tumor heterogeneity studies in lung cancer research
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The LCLC-97TM1 cell line has a doubling time of more than 50 hours.
The LCLC-97TM1 cell line is originated from a primary tumor in a 44-year-old man with a large cell lung carcinoma (LCLC), which is a type of non-small cell lung cancer (NSCLC). This cell line is used in cancer research to study the biology of lung cancer, including aspects like tumor growth, metastasis, and drug resistance.
The LCLC-97TM1 cell line has the following culture conditions:
Temperature: Incubate at 37°C
CO2 concentration: Incubate with 5% CO2
Medium: Exchange the culture supernatant at least every second day
Subculture: Split confluent cultures 1:3 to 1:5 once or twice a week using trypsin/EDTA
Seed out: Seed out at approximately 2×10^6 cells/80cm2
