NOMO-1

NOMO-1 is a human leukemia cell line established from the peripheral blood of a female patient with acute monocytic leukemia (AML), FAB M5a. These cells exhibit a promonocytic characteristics and can differentiate into macrophage-like cells by 12-o-tetradecanoyl phorbol-13-acetate (TPA) treatment. The cells show increased levels of fibronectin and the secretion of Interleukin-1 (IL-1) α and IL-1 β after TPA stimulation. TPA also induces the secretion of tissue factors from NOMO-1, and this secretion can be inhibited by warfarin. Additionally, plasminogen activator (PA) activity increases during a 17-day culture period. NOMO-1 cells are cultured in RPMI-1640 medium with 10% FBS at 37°C with 5% CO2, and they are cryopreserved at liquid nitrogen temperatures. This cell line is valuable for studying the differentiation and maturation of monocytes, especially in relation to acute monocytic leukemia and macrophage-like differentiation.