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Rat Mammary Epithelial Cells

ImageAcceGen cells & cell lines
Product Name

Rat Mammary Epithelial Cells

Cat.No

ABC-TC4166

Species

Rat

Biosafety Level

1

Size/Quantity

1 vial

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

Rat Mammary Epithelial Cells from AcceGen are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Mammary Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 110^6 cells and is delivered frozen.
Rat Mammary Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Mammary Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.
Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Recommended Medium And Supplement
Citation Guide

If the product is used for publication, it should be cited in the publication as: "AcceGen Cat.# XXX-0000". In addition, click here to submit your paper’s PubMed ID (PMID) to get a $100 coupon.

Application

For research use only

Product Type

Rat Primary Cells

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