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Rat Pulmonary Artery Smooth Muscle Cells

Product Name

Rat Pulmonary Artery Smooth Muscle Cells

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Cat.No

ABC-TC4203

Species

Rat

Size/Quantity

1 vial

Biosafety Level

1

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

Rat Pulmonary Artery Smooth Muscle Cells from AcceGen are isolated from tissue of Sprague-Dawley Rats. Rat Pulmonary Artery Smooth Muscle Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.
Cells are negative for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to α-smooth muscle actin.
Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by AcceGen.
Repeated freezing and thawing of cells is not recommended.
Standard biochemical procedures performed with smooth muscle cell cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Disease

Normal

Source Organ

Pulmonary Artery

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Recommended Medium And Supplement
Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

For research use only

Growth Conditions

37 ℃, 5% CO2

Cell Type

Smooth Muscle Cell

Growth Mode

Adherent

Product Type

Rat Primary Cells

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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