Rat Dermal Microvascular Endothelial Cells

Description

Rat Dermal Microvascular Endothelial Cells (RDMECs) are primary endothelial cells isolated from the microvasculature of the dermis in neonatal or juvenile rats. These cells form the inner lining of small blood vessels such as capillaries, arterioles, and venules, and are critical regulators of vascular permeability, angiogenesis, and inflammatory response in the skin. RDMECs display a classic cobblestone-like morphology under confluence and express key endothelial markers including CD31 (PECAM-1), von Willebrand factor (vWF), VE-cadherin, and endothelial nitric oxide synthase (eNOS). They also express angiogenesis-related molecules such as VEGFR-2 (Flk-1) and ICAM-1, and exhibit tube formation capability in extracellular matrix-based assays. RDMECs can be expanded for several passages in optimized endothelial culture media while maintaining stable marker expression and function. Repeated freeze-thaw cycles are not recommended. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.