Rat Ovarian Fibroblasts

Description

Rat Ovarian Fibroblasts (ROFs) are mesenchymal-derived cells primarily isolated from the ovarian stroma of rats. These cells are classified as stromal fibroblasts, playing a structural and functional role in maintaining ovarian tissue integrity. Morphologically, ROFs exhibit a spindle-shaped or elongated appearance with prominent cytoplasmic processes, typical of fibroblasts, and display a flattened, adherent growth pattern in vitro. ROFs contribute to ovarian ECM remodeling, cytokine secretion, and paracrine signaling, supporting folliculogenesis and steroidogenesis. Key molecular markers include vimentin and collagen type I. To ensure the quality of these primary cells, repeated freeze-thaw cycles are not recommended due to the fragile nature of these cells. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.