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| Product Code | M-07E; M-O7e; M07-e; M07e; Mo7e; MO7e; M07E; MO7E |
| Species | Human |
| Cat.No | ABC-TC1313 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Disease | Acute Megakaryoblastic Leukemia |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Leukemia Cell Lines |
The M-07e cell line is a human acute megakaryoblastic leukemia (AML M7) subline. It was established in 1987 from the peripheral blood of a 6-month-old Caucasian female patient and is derived from the M-07 parental line. M-07e cells rely on interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for proliferation. These cells exhibit responsiveness to a wide range of cytokines, encompassing GM-CSF, interferons (IFNs), interleukins (ILs), nerve growth factor (NGF), stem cell factor (SCF), tumor necrosis factor-α (TNF-α), and thrombopoietin (TPO). Prolonged culture may give rise to cytokine-independent subclones. In animal models, M-07e cells do not demonstrate tumorigenic or metastatic potential.
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M-07e cells provide a valuable model for studying AML M7, investigating its mechanisms and potential therapies. Additionally, M-07e cells are used to explore megakaryocytopoiesis and thrombopoiesis, particularly in relation to the effect of Thrombopoietin (TPO) on platelet production. Moreover, these cells contribute to understanding how cytokines impact cell proliferation, including studying the biochemical properties of interleukin receptors and post-ligand signaling pathways. Overall, M-07e cells contribute to research on AML M7, the role of TPO in megakaryocytopoiesis, and the effects of cytokines on cell growth and signaling pathways.
M-07E cells should be cultured in RPMI 1640 medium supplemented with 10-20% fetal bovine serum (FBS), 2mM L-glutamine, and 1% penicillin-streptomycin. Additionally, the medium should be supplemented with 10ng/mL GM-CSF, IL-3, or EPO to support growth. The cells should be maintained at 37°C in a humidified atmosphere with 5% CO₂.
Frequency: Passaging should be done every 2-3 days when the cells reach a density of 2-3×10⁶ cells/mL.
Procedure: Gently resuspend the cells by pipetting up and down.Transfer a portion of the cell suspension to a new culture flask with fresh complete medium supplemented with the appropriate growth factors.
Maintain the recommended cell density by adjusting the split ratio accordingly (usually 1:3 to 1:5).
Cryopreservation: Harvest and centrifuge cells at 300×g for 5 minutes. Resuspend cells in freezing medium (90% FBS and 10% DMSO) at a density of 1-2×10⁶ cells/mL. Aliquot the cell suspension into cryovials and gradually cool to -80°C using a controlled-rate freezing container before transferring to liquid nitrogen for long-term storage.
Thawing: Quickly thaw cryovials in a 37°C water bath. Transfer the cell suspension to a centrifuge tube containing pre-warmed complete medium. Centrifuge at 300×g for 5 minutes to remove DMSO. Resuspend the cell pellet in fresh medium supplemented with the appropriate growth factors and seed into culture flasks.
