Rat Microglial Cells

Description

Rat Microglial Cells (RMCs) are primary immune effector cells derived from the central nervous system (CNS) of rats. RMCs exhibit a highly branched, ramified structure under physiological conditions, characterized by a small soma, irregular nuclei, and multiple processes with spiny protrusions. RMCs maintain CNS homeostasis by clearing cellular debris, modulating neuroinflammation via cytokine secretion, and supporting synaptic pruning. Upon activation, they transform into an amoeboid phenotype with retracted processes, enlarged cell bodies, and enhanced phagocytic capacity. Key surface markers include ionized calcium-binding adapter molecule 1(Iba1) and TMEM119. These cells are non-proliferative in vitro. To ensure the quality of these primary cells, repeated freeze-thaw cycles are not recommended due to the fragile nature of these cells. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.