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Human Peripheral Blood Macrophages

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Peripheral blood Macrophages are derived from isolated peripheral blood CD14+ monocytes.
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Home | Primary Cells | Human Primary Cells | Blood System Primary Cells | Human Peripheral Blood Macrophages
Species

Human
Cat.No

ABC-H0081X
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Primary Cells
Size/Quantity

1 vial
Cell Type

Macrophage
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Peripheral Blood
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Blood Products

Description

Human Peripheral Blood Macrophages were derived from immunomagnetically selected peripheral blood monocytes. These monocytes are then cultured for 5 days in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), macrophage colony-stimulating factor (M-CSF), and interleukin 4 (IL-4) to differentiate them into macrophages. Macrophages play crucial roles in the immune system, participating in both innate and cell-mediated immunity. They are predominantly derived from peripheral blood monocytes, which are recruited from the bloodstream to various tissues in response to chemical signals from damaged tissues or chemotactic signals released by other cell types.

Human Peripheral Blood Macrophages

Why choose Human Peripheral Blood Macrophages from AcceGen?

Human Peripheral Blood Macrophages from AcceGen are a preferred choice due to their rigorous acquisition process. The cells are obtained using consent forms and protocols approved by the Institutional Review Board (IRB). Donors undergo comprehensive testing for HIV-1, HIV-2, hepatitis B, and hepatitis C, ensuring that the cells are obtained from individuals who tested negative for these viruses before donation.

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Citation

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Application

  • FOR RESEARCH USE ONLY

  • Human Peripheral Blood Macrophages play a pivotal role as a research tool due to the challenges associated with obtaining tissue macrophages in large quantities. They offer a valuable means to investigate various aspects of the immune system, macrophage-based therapies and macrophage polarization research. Additionally, these macrophages are utilized to explore the immunopharmacological activities of medicines, such as their immunostimulatory, anti-inflammatory, and antioxidant effects.

Frequently Asked Questions

  • What are Human Peripheral Blood Macrophages and how to get them?

    Human Peripheral Blood Macrophages are immune cells derived from peripheral blood monocytes. These monocytes are selected using immunomagnetic techniques and then cultured for five days in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), macrophage colony-stimulating factor (M-CSF), and interleukin 4 (IL-4) to differentiate them into macrophages.

  • What roles do macrophages play in the immune system?

    Macrophages play crucial roles in both innate and cell-mediated immunity. They are involved in the body’s defense against infections by engulfing and destroying pathogens, clearing dead cells, and releasing signals that activate other immune cells. They are predominantly derived from peripheral blood monocytes, which migrate to various tissues in response to chemical signals from damaged tissues or chemotactic signals released by other cell types.

  • How does AcceGen ensure the quality and safety of Human Peripheral Blood Macrophages?

    AcceGen ensures the quality and safety of Human Peripheral Blood Macrophages through a rigorous acquisition process. The cells are obtained using consent forms and protocols approved by the Institutional Review Board (IRB). Donors undergo comprehensive testing for HIV-1, HIV-2, hepatitis B, and hepatitis C to ensure the cells are obtained from individuals who tested negative for these viruses before donation.

  • Why are Human Peripheral Blood Macrophages from AcceGen preferred for research?

    Human Peripheral Blood Macrophages from AcceGen are preferred for research due to their rigorous selection and differentiation process, ensuring high quality and purity. The use of approved protocols and thorough donor screening for infectious diseases enhances the reliability and safety of the cells, making them suitable for various research applications in immunology and cell biology.

  • What are the key applications of Human Peripheral Blood Macrophages in scientific research?

    Human Peripheral Blood Macrophages are used in scientific research to study immune responses, the mechanisms of innate and cell-mediated immunity, and the role of macrophages in disease and health. They are valuable for investigating how macrophages respond to infections, clear dead cells, and communicate with other immune cells, which is critical for developing new therapies and understanding immune system functions

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