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Immortalized Mouse Dendritic Cell Line | ||||
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Product Name | Immortalized Mouse Dendritic Cell Line | |||
Price | Get Quote | |||
Cat.No | ABI-TC329D | Species | Mouse | |
Size/Quantity | 1 vial | Biosafety Level | 2 | |
Shipping Info | Dry Ice | Storage | Liquid Nitrogen | |
Description | As messengers bridging the innate and adaptive immune system, the conventional tissue-resident DC (cDC) acts as sentinels in secondary lymphoid organs and other tissues for antigen capture and presentation. The Wild-type MutuDC1940 cell line is an Immortalised splenic CD8α+ subset (CD11chigh,B220-,DEC205+,CD24high,CD11b-) of conventional dendritic cells derived from the CD11c:SV40LgT transgenic mice. The MutuDC1940 cell line retains response to TLR ligands such as CpG (TLR9-L) and PolyIC (TLR3-L) and to a lesser extent LPS (TLR4-L) stimulation by up-regulation of co-stimulatory molecules CD40, CD80 and CD86. In addition to responding to PAMP stimulation and producing Th1 cytokines such as IL-12, these cells are also capable of presenting antigen in the context of both MHC-I and MHC-II, including direct antigen presentation and cross-presentation of cell-associated antigens. | |||
Disease | Normal | |||
Source Organ | Lymphoid | |||
Quality Control | 1) Direct antigen presentation and cross-antigen presentation were evaluated by the MHC-I (SIINFEKL/OT-I ) and MHC-II (OVA323-339/OT-II) restricted systems; 3) proteome profile and surface markers assessed by RT-PCR; RT-PCR; 3) IL-12 cytokine secretion analyzed using ELISA, 4) Response to PAMP stimulation evaluated by functional assays. | |||
Recommended Medium And Supplement | ABI-TM329D Mouse Dendritic Cell Line Medium Kit | |||
Citation Guide | When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). | |||
Application | For research use only | |||
Morphology | Small aggregates | |||
Immortalization Method | Isolated from C57BL/6 transgenic mice carrying SV40 Large T oncogene | |||
Growth Conditions | 37 ℃, 5% CO2 | |||
Cell Type | DC Cell | |||
Growth Mode | Adherent | |||
Product Type | Immortalized Cell Line | |||
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