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Rat Mammary Epithelial Cells

Cat.#ABC-TC4166
TypeRat primary cells
SpeciesRat
Biosafety Level1
Size1 vial
Storageliquid nitrogen
ShippingDry Ice
Related Medium infoComplete Epithelial Cell Medium
DescriptionRat Mammary Epithelial Cells from AcceGen Biotech are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Mammary Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen Biotech’s Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells and is delivered frozen. Rat Mammary Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Mammary Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by AcceGen Biotech. Repeated freezing and thawing of cells is not recommended. Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
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