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Specieshuman- 60 years, M
Storageliquid nitrogen
Shippingdry ice
Growth Propertiescontinuous culture, grown as monolayer. incubate with trypsin/EDTA at 37C for at least 10-15 min. After thawing the viability of the cells may be low.
Recommended MediumMEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids; split confluent cultures 1:2-1:3 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2
DescriptionTissue: pancreas; Tumor: adenocarcinoma; Derived from ascitic fluid. Karotype: modal number 77. CEA and CA19-9 production
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