Human B Cells-Negatively Selected

Description

Human B Cells-Negatively Selected are primary lymphocytes isolated from human peripheral blood through stringent negative selection protocols, which are central to studying negative selection of immune cells and B cell negative selection pathways. These cells are cryopreserved immediately after isolation to preserve their native immunological properties without a specific disease association. They exhibit characteristic small, round morphology in suspension cultures. Functionally, they are pivotal in the adaptive immune response, recognizing antigens, differentiating into plasma cells, and secreting antibodies to neutralize pathogens, with their malfunction linked to immune-related disorders like lupus. These human cells express characteristic surface markers CD19 and CD20, and have limited expansion capacity. Repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.