Human C. Difficile Peripheral Blood Mononuclear Cells

Description

Human Clostridioides difficile infection (CDI) peripheral blood mononuclear cells (PBMCs) are isolated from peripheral blood of patients with clinically confirmed C. difficile-associated diarrhea (CDAD) or colitis, providing critical insights into the host immune response to this nosocomial pathogen. These human-derived PBMCs contain immune cell populations—including Th17 cells, regulatory T cells, and activated monocytes—that exhibit dysregulated mucosal immunity, characterized by exhausted T-cell phenotypes (PD-1⁺/LAG-3⁺) and impaired Th17/IL-17 responses, which correlate with disease severity and recurrence. The cells exhibit typical mononuclear morphology with intact surface markers and maintain robust functional responses to bacterial antigens, reflecting the complex interplay between gut microbiota disruption, antibiotic exposure, and systemic immunity in CDAD pathogenesis. These PBMCs are particularly valuable for studying toxin-mediated immune modulation and vaccine development against this increasingly antibiotic-resistant pathogen.